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lncRNA Sequencing

Service description:
rRNA within total RNA are depleted for enrichment of mRNA and lncRNA transcripts. Enriched fragmented RNA are subsequently converted to cDNA for library preparation.
After that cDNA libraries are sequenced using Illumina PE150 bp sequencing strategy.

Sample requirement:
Total RNA ≥ 500ng
Concentration ≥ 25ng/μl
RIN ≥ 6.5
A260/280 = 1.8-2.2

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LncRNA sequencing is a high-throughput transcriptomic method used to identify, quantify, and analyze long non-coding RNAs (transcripts longer than 200 nucleotides that do not encode proteins).


Key Applications:

  • Cancer Research: Identifies tumor-specific lncRNAs and aberrant regulatory pathways involved in cancer progression, metastasis, and therapy resistance.

  • Disease Biomarker Discovery: Uncovers condition-specific lncRNA expression patterns to serve as novel diagnostic, prognostic, and patient stratification markers.

  • Developmental Biology: Maps and tracks lncRNAs that regulate cell differentiation, tissue-specific developmental peaks, and complex embryonic pathways.

  • Neurobiology: Explores intricate gene-regulation networks underlying brain development and neurodegenerative conditions.

  • Agricultural and Animal Breeding: Applied in genetic improvement and trait selection, such as detecting quantitative trait markers or lipid profiles in livestock.

  • Drug Discovery: Pinpoints targetable regulatory lncRNAs to design novel therapeutic interventions or RNA-modulating drugs.

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