Nanopore Platform

Direct mRNA Sequencing:

· No reverse transcription and no amplification steps, full-length transcript is directly sequenced by Nanopore platform.

· Methylation modification sites such as m6A, m5C and psudouridine can be detected.

· Other transcriptomic profiles such as alternative splicing, fusion gene and new isomers can also be analyzed.

Whole Genome Bisulfite Sequencing:

With advantages of high throughput and long read, combined with unique technology of Nanopore, methylation information within the whole genome can be captured without the need of bisulfite processing beforehand.

Whole Plasmid Sequencing:

Whole plasmid sequencing is our end-to-end solution for cost-effective, rapid, and high-throughput sequencing of entire plasmids. Unlike conventional methods that only sequence selected regions, ONT  verifies the integrity and fidelity of whole plasmids using long-read sequencing. This ensures the detection of unwanted changes, providing a complete plasmid map and preventing potential replication issues.

Deliverables:

1. FastQ files

2. Read-length and quality report

3. Consensus plasmid assembly

4. Plasmid annotation files

5. Assembled sequence in .ab1 format

6. Variant calling result in VCF file